Abstract
The whole-body autoradiographic technique involves a series of animals, such as mice, which are injected intravenously with the same dose of a labeled compound. At intervals, the animals are rapidly frozen and sagittal sections are taken at the different levels of interest with a cryostat microtome. The sections are freeze-dried and pressed against a photographic film. After suitable exposure, section and film are separated and the film is developed. The distribution pattern of the substance and its metabolites appear on the films—the autoradiograms. The sections may be stained and mounted under a cover glass, or they may be used in their unstained state as references for the interpretation of the autoradiograms. Quantitative data concerning the radioactivity in different tissues can be obtained by impulse counting of pieces taken from the sections collected on tape, or by densitometry using an isotope scale as a reference source. Tissue pieces may also be punched out from the sections for the microseparation of metabolites. By the use of different survival times, the variation in distribution can be followed as a function of time.
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