Abstract
This chapter discusses the preparations and manipulations preceding electron paramagnetic resonance (EPR) spectroscopy proper so that results of the desired precision and decisiveness are obtained. Preparation of samples for EPR spectroscopy differs sufficiently from general practice in other methods used in biochemistry. The main reason for different requirements stems from the geometry of the sample container, which is dictated by the spectroscopic procedure, and from the relatively low sensitivity of EPR spectroscopy. As most features observable by EPR spectroscopy in the field of oxidoreduction and of electron-transfer systems requires low temperature spectroscopy, the discussion deals with procedures suitable for low temperature work. Procedure for filling EPR tubes aerobically and anaerobic procedures for sample preparation are described in the chapter. In addition combination of low-temperature EPR with optical spectroscopy and Mossbauer spectroscopy is presented.
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