Abstract
This chapter describes a technique to obtain a non-T, non-B, radio-resistant, glass-adherent cell population from mouse spleen cells. This splenic adherent cell (SAC) population fulfills both the accessory cell and antigen-presenting cell requirements of primary and secondary in vitro antibody responses. An essential requirement for the function of a non-T, non-B adherent cell population has been demonstrated in a number of immune response systems. This chapter describes a technique that isolates adherent accessory or macrophage-like cells (SAC) from mouse spleen cells on the basis of their radio-resistance and adherence properties. The viable cell recovery of the adherent, radio-resistant, T cell-depleted SAC populations after overnight incubation is approximately 1% of the initial spleen cell population. The cell fractionation procedure is consistently successful in isolating the non-T, non-B, radio-resistant, adherent cell population present in mouse spleen cells that provides accessory cell function in a number of immune response systems.
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