10-Hydroxy-trans-2-decenoic acid attenuates angiotensin II-induced inflammatory responses in rat vascular smooth muscle cells

Abstract

10-Hydroxy-trans-2-decenoic acid (10-HDA) is a major medium chain fatty acid in royal jelly. In this study, the rat vascular smooth muscle cells (VSMCs) were pretreated with 10-HDA (250 μM, 500 μM, 1000 μM), followed by incubation with Angiotensin II (Ang II, 100 nM). 10-HDA significantly reduced the productions of Ang II induced inflammatory cytokines interleukin 6 and C-reactive protein, through downregulating the nuclear factor kappa B (NF-κB) and mitogen-activated protein kinase (MAPK) signaling pathways. 10-HDA also attenuated the Ang II-stimulated reactive oxygen species production and the NADPH oxidase 4 (NOX4) expression. Further mechanistic studies showed that 10-HDA reduced the elevated abundances of the angiotensin II type 1 receptor, and inhibited cAMP-response element-binding protein binding to the cAMP-response element site in the IL-6 promoter region. The results suggested that 10-HDA effectively attenuated Ang II-induced inflammatory responses and ROS production via regulating multiple steps in NF-κB and MAPK signaling pathways in VSMCs.