Abstract
Random amplified polymorphic DNA (RAPD) analysis was used to amplify the genome of giant tiger prawn (Penaeus monodon) to detect DNA markers and investigate genetic variation in wild black tiger prawn from coastal West Bengal in India. A total of eighty 10-base primers were screened, and 20 primers yielded amplification products. Three positive primers were selected to analyze three geographically different samples of coastal P. monodon from West Bengal. A total of 40 reproducible RAPD fragments ranging in size from 100 to 1000bp were scored and 20 fragments (50%) were found to be polymorphic. The RAPD analysis of juveniles from three different locales, Digha coast, North 24 Parganas, South 24 Parganas, revealed different levels of genetic variability among the samples. The percentages of polymorphic bands in North 24 Parganas and South 24 Parganas suggested a high genetic variability among the samples. Digha coast indicated lower polymorphic levels among three samples. Population specificity was also found within the three samples.
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