Abstract
Ion-selective microelectrodes (ISMs) provide an unparalleled tool for the simultaneous characterization of electrophysiologic and ionic properties of identified cells within functioning tissue. A simultaneous measurement of ionic activities and cell electrical properties remains a feat unmatched by any single tool for measuring bioelectric events or ionic species. A dramatic improvement in pH ISM technology came with the development of liquid membrane pH ISMs based on tridodecylamine (TDDA). Intracellular ISMs based on neutral carriers offer distinct advantages over glass ISMs. Liquid membrane ISMs are considerably easier to fabricate, generally more selective for principal ions, and respond faster than their glass counterparts. Indeed, pH ISMs based on TDDA, a neutral carrier that is highly selective for protons, are the easiest to fabricate among pH ISMs. They are the fastest, finest-tipped, and most selective double-barrel pH-sensitive ISMs fabricated to date. TDDA-based pH ISMs have become the microelectrode of choice for studying pH i changes from individual brain cells within intact tissues.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
