Abstract
Publisher Summary Epidermal growth factor (EGF) is a single chain polypeptide containing amino acid residues that exhibits potent mitogenic activity for a variety of cell types both in vivo and in vitro . Many reviews are available concerning the mechanism of action and biological effects of EGF. This molecule was first described by Cohen, who isolated EGF from the submaxillary glands of adult male mice (mEGF). Since then, EGF has been isolated from rat, guinea pig prostate, and human urine (hEGF). This chapter discusses a part of the standard published procedure and presents the use of monoclonal antibody affinity chromatography in the light of other published reports using this technology.
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