Abstract
High-performance liquid chromatography (HPLC) has been extensively utilized in separation of a number of glycosphingolipids. The quantity of the glycolipid mixture to be separated, the size of the column, the conditions of elution, particularly the composition and slope of gradient elution, and the speed of elution can be varied from one case to another. This chapter describes the general rules of the method applied for separation of different types of glycolipids by modifications of this method. However, glycolipids with close chromatographic mobilities are coeluted and cannot be separated by a simple application of this method. The conditions under which these components can be separated as acetylated derivatives are also described in the chapter. One procedure for the separation of different types of glycolipids is the separation of underivatized glycolipids by high-performance liquid chromatography. Intact glycosphingolipids without derivatization can be readily separated by HPLC with elution by gradient solvents composed of isopropanol–hexane–water in various ratios.
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