Abstract
The objective of this study was to identified the development of the immunoreactive follicle stimulating hormone (ir-FSH) cells in pituitary glands of rats (Rattus norvegicus) in different age using immunohistochemical (IHC) methods. The pituitary organ that used in this study was collected from eight white female rats aged 2, 4, 6, and >12 months. Pituitary glands were processed histologically and stained with IHC methods to detect the ir-FSH cells. The result showed that the ir-FSH cells were observed in pars distalis (PD), pars tuberalis (PT), and pars intermedia (PI) of adenohypophysis with different number of cells. Furthermore, ir-FSH cells were found abundantly (+++) in the PT and a few (+) in PD of rats aged two months. In rats aged 4 months those cells were distributed in slight number (+) in PT and abundant (+++) in PD. In addition, the cells were also found in a great quantities in rats whose ages were 6 months, (+++) in both PT and PD, but these cells decreased (++) in rats aged >12 months in PD and absent (-) in PT. In conclusion, the devalopment and distribution of ir-FSH cells were found in pars tuberalis and pars distalis adenohypophysis in the pituitary glands of rats aged 2 up to >12 months with different number and distribution patterns.
Highlights
Organ endokrin merupakan kelenjar tanpa duktus yang mensintesis hormonhormon yang disekresikan ke dalam sirkulasi darah untuk di transportasikan ke seluruh tubuh menuju organ target
Peran follicle stimulating hormone (FSH) lainnya adalah sebagai regulator siklus estrus bersama luteinizing hormone (LH) untuk merangsang perkembangan sel-sel folikel ovarium, selsel granulosa, dan sel-sel teka dalam mensekresikan hormon estrogen
Instruction For Immunohistochemical Staining Using Goat Primary Antibodies. http://www.vectorlabs.com. (27 November 2015)
Summary
Pengambilan kelenjar hipofisa tikus Sampel yang digunakan dalam penelitian ini adalah organ hipofisa dari tikus putih (Rattus norvegicus) betina berumur 2 bulan, 4 bulan, 6 bulan, dan >12 bulan. Organ hipofisa yang diperoleh selanjutnya dicuci dengan larutan NaCl. Patrick Flaggellata, dkk fisiologis 0,9 % dan dimasukkan ke dalam larutan fiksatif paraformaldehid 4% selama 48 jam dan dipindahkan ke dalam alkohol 70% sampai dilakukan proses dehidrasi. Tahap berikutnya adalah infiltrasi jaringan dalam parafin cair I, II, dan III pada temperatur 600C masingmasing selama 1 jam dan dilanjutkan dengan proses penanaman jaringan di dalam parafin (embedding). Selanjutnya preparat jaringan (slide) dibilas dengan larutan TBS dan diinkubasi dengan normal horse serum. Tahap berikutnya adalah inkubasi slide dengan antibodi primer (anti FSH) dan dimasukkan ke dalam refrigerator pada temperatur 40C selama 1 malam (overnight). Slide dibilas kembali dengan TBS lalu diinkubasi dengan antibodi sekunder dalam inkubator temperatur 370C selama 30 menit dan dicuci dengan TBS. Selanjutnya slide dibilas dengan akuades dan dilanjutkan dengan proses pewarnaan latar jaringan (counterstain) menggunakan larutan. Tahap terakhir adalah proses dehidrasi, clearing, dan mounting dengan bahan perekat Entellan®
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