1/f-Noise of open bacterial porin channels.

Abstract

General diffusion pores and specific porin channels from outer membranes of gram-negative bacteria were reconstituted into lipid bilayer membranes. The current noise of the channels was investigated for the different porins in the open state and in the ligand-induced closed state using fast Fourier transformation. The open channel noise exhibited 1/f-noise for frequencies up to 200 Hz. The 1/f-noise was investigated using the Hooge formula (Hooge, Phys. Lett. 29A: 139-140 (1969)), and the Hooge parameter alpha was calculated for all bacterial porins used in this study. The 1/f-noise was in part caused by slow inactivation and activation of porin channels. However, when care was taken that during the noise measurement no opening or closing of porin channels occurred, the Hooge Parameter alpha was a meaningful number for a given channel. A linear relationship was observed between alpha and the single-channel conductance, g, of the different porins. This linear relation between single-channel conductance and the Hooge parameter alpha could be qualitatively explained by assuming that the passing of an ion through a bacterial porin channel is-to a certain extent-influenced by nonlinear effects between channel wall and passing ion.