Abstract

Substrate activity of 1- and 3-deazapurines in the reaction of microbiological ribo- 2-deoxyribosylation catalysed by purine nucleoside phosphorylase of viable cells ofE. coli BMT-1D/1A has been studied. Guanosine or 2′-deoxyguanosine were used as donors. 1-Deazapurines are good substrates in both reactions; 3-deazapurines are very effective intransdeoxyribosylation but not intransribosylation. Benzimidazole is an excellent substrate in both reactions indicating that N(1) and N(3) are not essential for transglycosylation.

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