Abstract
Gliomas are the most common primary neoplasms of the central nervous system (CNS) in adults. The powerful tumor invasiveness is closely related to tumor angiogenesis, tumor-associated macrophages and hypoxia. Studies indicate that stroma-derived factor -1 (SDF-1), one of tumors-secreted angiogenesis-related factors, affects tumor growth rate and tumor invasion capability. In this study, we used mouse astrocytoma cells, ALTS1C1, as a research model to over-express sdf-1 gene in order to establish an invasive tumor model and study the tumor microenvironments of an invasive astrocytoma. Firstly, the ALTS1C1-SDF-1 cell line was established by using transgenic DNA liposome method. Secondly, the relationship between SDF-1 and tumor cell migration, proliferation, tumor microvessel density, or tumor -associated macrophages was studied by immunohistochemical (IHC) apprach. Finally, the relative change of microglia and macrophage during tumor progression was examined by flow cytometery. This study has successfully established an invasive brain tumor model and provided evidences to demonstrate that SDF-1 acts as a chemoattractant or survival factor for TAM tropism toward hypoxic tumor region. This brain tumor model serves an important tumor model for the design of new treatment protocol against the invasive glioma.
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