Abstract

Summary A cell-free system prepared from Citrus sinensis exocarp transformed R -[2- 14 C]-mevalonic acid into abscisic acid, phaseic acid and 1′,4′- trans -abscisic acid dioL 1′,4′- Trans -abscisic acid diol was unequivocally characterized as the major product of mevalonic acid metabolism in the Citrus cell-free system. The addition of (R,S)-abscisic acid as a «cold-pool trap» increased incorporation of radioactivity into putative abscisic acid but reduced the levels of [ 14 C] associated with 1′,4′- trans -abscisic acid diol, negating a role for this metabolite as a precursor to abscisic acid.

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