Abstract
1α,25-dihydroxyvitamin-D3[1α,25(OH)2D3] is known to potentiate in osteoblast-like rat osteosarcoma cells (ROS 17/2.8) the influx of Ca2+through voltage-activated L-type Ca2+channels. In the present study, by means of the whole-cell patch-clamp technique, we found that physiological concentrations of 1α,25(OH)2D3(5–5000 × 10−11M) also stimulate, in a dose-dependent manner, outwardly rectifying anionic currents over the course of 1–5 minutes. These currents, recorded in the presence of 130 mM glutamate or ≈140 mM Cl−in the external solution, were blocked by 200 μM DIDS, a stilbene derivative selective for Cl−channels. We conclude that, in addition to the already known effects of 1α,25(OH)2D3on Ca2+channels, the hormone also enhances in ROS 17/2.8 cells an outward anion conductance. This is the first report concerning activation of anion channels by 1α,25(OH)2D3.
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