Abstract
The purpose of these studies was to investigate the 1,25‐dihydroxyvitamin D (1,25D) regulation of breast epithelial cell lipid metabolism and breast to bone metastasis. The untransformed, MCF10A (10A), and metastatic, MCF10CA1a, breast epithelial cells were employed for these studies. Lipid accumulation was greater in MCF10CA1a cells compared to 10A cells as shown by oil red O staining, thin layer chromatography and colorimetric triglyceride analysis. Following 1,25D treatment (10nm, 48 hrs) of MCF10CA1a cells, the mRNA expression of lipid metabolism regulators fatty acid synthase (FAS), insulin inducing gene 2 (Insig2), and perilipin 2 (PLIN2) were significantly increased while carnitine palmityoltransferase 1 (CPT1a) was significantly decreased compared to vehicle. The impact of 1,25D on breast to bone metastasis was assessed employing a unique 3‐dimensional transwell matrigel invasion assay with MCF10CA1a cells. Results showed that fewer 1,25D treated cells implanted in the bone matrix compared to vehicle treated cells. In addition, 1,25D treated cells in the bone matrix retained an epithelial‐like phenotype compared to the mesenchymal‐like phenotype of the vehicle‐treated cells. These results suggest that 1,25D may regulate lipid metabolism and inhibit breast to bone metastasis.Grant Funding Source: supported by NCI CCSG CA23168
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