実験的脂肪肝にかんする生化学的研究第1篇 実験的脂肪肝の脂質分析

Abstract

In an attempt to determine serum and liver lipid fractions, thioctic acid (TA) and tri-phenyl-ethanol derivatives (TPE) were administered to an experimental fatty liver in rats due to CCl4, and thioctamide(TAM), TPE, orotic acid(OtA)and pangamic acid(PgA)were administered to an experimental fatty liver in rabitts. The results obtained were as follows:1. Following the administration of TA and TPE to the CCl4 fatty liver the TA administered group showed a more decrease of total cholesterol, ester cholesterol, total fatty acid and total fat in liver lipid fractions, than the TPE group, But the TPE administered group showed a more decrease of neutoral fat and a more increase of lecithin in phospholipid than the TA administered group.2. Following the administration of TAM, OtA, TPE and PgA to the cholesterol fatty liver, the highest decrease of liver lipid fractions was observed in total cholesterol and ester cholesterol in the OtA administered group, followed by the PgA, TPE administered groups in order; of total fatty acid in the TPE administered group, followed by the PgA, OtA and TAM administered groups in order, of total fat in the TPE administered group followed by the PgA, OtA and TAM administered groups in order. An increase in lecithin in phospholipid was the highest in the TPE administered group followed by the PgA, TAM and OtA administered groups in order; a decrease in cephalin was the highest in the TAM administered group followed by the OtA PgA and TPE administered groups in order; sphingomyelin showed an increase in the OtA and the PgA administered groups but decrea sed in the TAM and TPE administered groups.3. Following the administration of TAM, TPE, OtA and PgA to the cholesterol fatty liver, total cholesterol in serum lipid fractions showed the highest decrease in the TPE administered groups, followed by the OtA, TAM and PgA administered groups in order; of ester cholesterolin the TPE administered group, followed by the TAM, OtA and PgA adminis tered groups in order; of total fatty acid in the OtA administered group followed by the TPE, TAM and PgA administered groups in order; of neutral fat in the OtA administeredgroup followed by the TPE, PgA and TAM administered groups; of total fat in the TPE administered group followed by the OtA, TAM and PgA administered groups in order An increase of lecithin in phospholipid was the highest in the TAM administered group, followed by the OtA, TPE and PgA administered groups in order. cephalin showed no changes in the OtA administered group, but it was most decreased in the TPE administered group, followed by the TAM and PgA administered groups. Sphingomyelin showed no changes in the OtA administered group, but it was decreased in the PgA, TPE and TAM administered groups.4. On the basis of the result of 1 the TA administered group showed a more favorable lipotropic action against liver lipid fractions in CCl4 fatty liver than the TPE administered group.5. On the basis of the result of 2, so far as medication efficacy was concerned, the OtA administered group showed a favorable lipotropic action for total cholesterol and its fractions; so did the TPE administered group for neutral fat and total fat. The TPE administered group showed the highest increase in lecithin and a decrease in cephalin.6. On the basis of the result of 3, it was disclosed that the TPE administered group showed a favorable lipotropic action for cholesterol fractions; so did the OtA administered group it for neutral fat. The TAM administered group showed a marked increase in lecithin and a decrease in cephalin.