090 Long noncoding RNA expression profile in monocyte-derived dendritic cells from patients with systematic lupus erythematosus

Abstract

There is increasing interest in the potential involvement of lncRNAs in SLE etiology, owing to aberrant function of lncRNAs in cell proliferation, differentiation, apoptosis, development and immune responses. Aberrant expression of lncRNAs affect the function of moDCs and thus influence clinical diseases. The aim of our study was to explore lncRNA expression in moDCs of SLE patients to provide new insight into the pathogenesis of SLE. LncRNA and mRNA microarrays were performed to identify differentially expressed lncRNAs in moDCs of SLE patients compared with normal controls. QPCR was used to validate the results and correlation analysis was used to analyze the relationship between these aberrantly expressed lncRNAs and SLEDAI score.163 lncRNAs were differentially expressed in moDCs between SLE and normal controls, including 118 upregulated and 45 downregulated. A total of 137 mRNAs were differentially expressed in moDCs of patients with SLE, including 83 upregulated and 54 downregulated. Furthermore, qPCR data showed that lncRNA ENST00000604411.1 (18.23-fold, P < 0.001) and ENST00000501122.2 (1.96-fold, P < 0.001) was up-regulated and the other two lncRNAs lnc-HSFY2-3:3 (0.42-fold, P < 0.001) and lnc-SERPINB9-1:2 (0.50-fold, P = 0.040) were down-regulated in moDCs of SLE patients. The expression level of ENST00000604411.1 (r=0.593, P=0.020) and ENST00000501122.2 (r=0.539, P=0.038) was positively correlated with the SLEDAI score respectively.The results indicate that the dysregulation of lncRNAs may be involved in the pathological processes of moDCs in SLE patients. The expression level of ENST00000604411.1 and ENST00000501122.2 may have potential value for the assessment of the disease activity of SLE.