0853. Elevated levels of soluble rage predict impaired alveolar fluid clearance in a translational mouse model of acute respiratory distress syndrome (ARDS)

R Blondonnet,D Bouvier,G Marceau,G Clairefond,J Audard,J-M Constantin,V Sapin,M Jabaudon,P Dechelotte,P Blanc

doi:10.1186/2197-425x-2-s1-p62

Abstract

Receptor for advanced glycation endproducts (RAGE) is a transmembrane pattern-recognition receptor of the immunoglobulin superfamily that is abundantly expressed in the lung and primarily located on the basal surface of alveolar type I cells. RAGE is implicated in ARDS as an important pathway to alveolar inflammation and, when its soluble form sRAGE is assayed in plasma or pulmonary edema fluid, as a marker of AT I cell injury [1]. Functional activity of AT 1 cells can be assessed by the measurement of alveolar fluid clearance (AFC) rate [2], but the relationship between sRAGE plasma levels of sRAGE and AFC rates has never been investigated.

Highlights

Receptor for advanced glycation endproducts (RAGE) is a transmembrane pattern-recognition receptor of the immunoglobulin superfamily that is abundantly expressed in the lung and primarily located on the basal surface of alveolar type I cells
Functional activity of AT 1 cells can be assessed by the measurement of alveolar fluid clearance (AFC) rate [2], but the relationship between sRAGE plasma levels of sRAGE and AFC rates has never been investigated
Before initiation of MV, 1R2D2 - EA 7281, School of Medicine, Universite d’Auvergne ClermontFerrand I, Clermont-Ferrand, France Full list of author information is available at the end of the article all mice received a tracheal instillation of bovine serum albumin (BSA 5%) in order to detect changes in alveolar protein levels over 30 minutes