078 The effect of LPS on neutrophil extracellular trap-induced Th17 polarization is IL-6 and STAT3-Dependent

Abstract

Using a 7-day in vitro assay involving CD3-CD28 stimulation of peripheral blood mononuclear cells (PBMC), we have previously reported that neutrophil extracellular traps (NETs) exert a stimulatory effect on Th17 polarization from pre-existing memory T-cells (JID 139:125) and that the stimulatory effect of NETs is monocyte-dependent (JID 139:S69). To further investigate the nature of this response, we carried out single-cell RNA-seq analyses, confirming a large increase in the numbers of IL-17-expressing CD4+ T-cells between day 2 and day 7 cultures, which was enhanced by NETs.