068 Structural and Biochemical Characterization of Renal Capsule Matrix (RCM) Biomaterial

Abstract

Cellular interaction with biomaterials determines the fate of the implant. Naturally derived biomaterials such as small intestinal submucosa (SIS) and renal capsule matrix (RCM) may provide optimal structure and composition for cellular repopulation in certain wound healing applications. Porcine renal capsule matrix (RCM) is an acellular, naturally occurring extracellular matrix undergoing characterization and preclinical investigation for the treatment of soft tissue injury. Early studies have demonstrated that RCM constructs induce host tissue infiltration, tissue-specific remodeling, and repair. RCM consists primarily of collagen, although the other proteins have been detected, including the growth factors basic FGF, VEGF, and CTGF. Transmission and scanning electron microscopy were used to envision the cellular environment provided by this biomaterial. Comparison of the images utilizing cryo and traditional preparation techniques show that RCM is a dense, heterogeneous matrix consisting of textured fibers of mixed size. Although the matrix architecture shows signs of collapse following lyophilization, much of the native structure seen in the hydrated isolate is preserved. These differences are maintained following rehydration in normal saline. The effects of these structural changes on cellular reaction and infiltration are unknown, but the naturally complex architecture likely facilitates host interaction at the cellular level. Further compositional characterization is under way. This work was supported by Cook Biotech Incorporated, West Lafayette, IN.