062 Phage display-based isolation of collagen VII-specific monoclonal antibodies from mice with actively induced Epidermolysis Bullosa Acquisita

Abstract

To establish an entirely murine antibody transfer model for Epidermolysis Bullosa Acquisita (EBA) research, we generated a defined array of monoclonal antibodies (mAbs) against the second von Willebrand factor type A-like domain of murine collagen VII (mCOL7vWFA2), the immunodominant epitope in EBA. To identify mCOL7vWFA2 binding antibodies, we generated two single-chain fragment variable (scFv) immune libraries from the spleen and inguinal lymph nodes of a mCOL7vWFA2 immunized mouse with active EBA disease, confirmed by disease affected body area (10%) and anti-mCOL7vWFA IgG serum titer.