Abstract
The study of the changing levels of microRNAs (miRNAs) in different biological body environments as diagnostic and prognostic marker for ST-elavation myocardial infarction (STEMI) calls attention of scientists. We conducted a research to determine the level of several microRNAs in blood plasma, isolated monocytes and platelets of STEMI pts in the dynamics of disease development (the 1st and the 7th days). Into the study we included the evaluation of some cardiac microRNAs (1, 208a, 208b, 499), inflammation-related and hypoxic-response microRNA-155 and –210. 20 STEMI pts (mean age 54,85±2,2 years) were identified in National Scientific Center Institute of Cardiology M.D. Strazhesko. The control group included 10 subjects of comparable sex and age without any evidence of coronary artery disease. Expression miRNAs was quantified from plasma, isolated monocytes and platelets using quantitative Real-Time polymerase-chain reaction system. In plasma the microRNA-1 level was significantly higher only on day 7, the microRNA-155 level was lower on the first day and 15 times higher on day 7. In platelets the microRNA-1 level was 4.2 times higher on the first day of STEMI in comparison with the control group and on day 7 of STEMI returned to the level of the control group. The changes in monocytes were more revealing: the microRNA-1 level was higher by 28 times on the first day of STEMI, the microRNA-208a level was higher by 2 times on day 1 and by 19 times on day 7 of STEMI, and the microRNA-155 level was higher by 17 times on day 1 and by 260 times on day 7 of STEMI in comparison to the control group. The miRNA distribution is unique amongst blood plasma, isolated monocytes and platelets in pts with STEMI. Our results show higher informational content of evaluation of certain miRNAs in isolated cells comparing with blood plasma, and expresses the necessity of determining miRNA levels in the dynamics of STEMI.
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