(051) METHAMPHETAMINE-INDUCED VAGINAL LUBRICATION IN RATS AND THE ESTROUS CYCLE

Abstract

Abstract Introduction In an interview-based study performed by our lab of women who use methamphetamine (meth), we previously found that the drug, when delivered intravenously (IV), produces a feeling indistinguishable from an orgasm, which is accompanied by a flushing sensation and the production of excessive vaginal lubrication. We have also reported meth-induced vaginal lubrication in an anesthetized animal model. This production of vaginal transudate was dependent on the dose of meth used and the potent vasodilator nitric oxide. Objective The objective of the current study was to assess meth-induced vaginal lubrication across different stages of the estrous cycle and following ovariectomy (ovx) in anesthetized rats, and to assess plasma levels of two signaling molecules associated with sexual arousal. Methods Anesthetized rats, each previously implanted with a chronic indwelling jugular catheter, were anesthetized and given one IV infusion of meth (0.24 mg/kg) and vaginal lubrication was measured. A pre-weighed cotton-tipped swab, inserted into the vaginal canal, collected fluid secreted following the administration of meth. Blood was withdrawn before and at ten and fifteen minutes after meth administration either via the jugular catheter or via a small tail snip to measure plasma levels of estradiol and nitric oxide. Testing was completed at each stage of the estrous cycle as well as before and after ovariectomy. Results Estradiol and nitric oxide increased following meth infusion similarly across all stages of the estrous cycle. Vaginal lubrication increased more with meth treatment than with saline treatment. This was true across all stages of the estrous cycle and despite ovariectomy. Conclusions These findings have far-reaching implications for those who suffer from vaginal dryness. The mechanism underlying this phenomenon may present a novel pharmacotherapeutic target to treat vaginal dryness. Since meth still produced vaginal secretions in anesthetized rats, these results suggest that targeting this mechanism may even prove beneficial in post-menopausal women, a patient group likely at highest risk for experiencing vaginal dryness. Disclosure Any of the authors act as a consultant, employee or shareholder of an industry for: Embera Neuurotherapeutics & JanOne.