Abstract
The capability to perform high-resolution, wide field-of-view (FOV) microscopy imaging is highly sought after in biomedical applications. In this paper, we report a wide FOV microscopy system that uses a closed-circuit-television (CCTV) lens for image relay and a flatbed scanner for data acquisition. We show that such an imaging system is capable of capturing a 10 mm × 7.5 mm FOV image with 0.78 µm resolution, resulting in more than 0.5 billion pixels across the entire image. The resolution and field curve of the proposed system were characterized by imaging a USAF resolution target and a hole-array target. To demonstrate its application, 0.5 gigapixel images of histology slides were acquired using this system.
Highlights
The conventional microscope architecture generally consists of a microscope objective for light collection from a sample slide, intermediate relay optics, and paired or single eyepieces that project a magnified image of the sample into the eyes
A new method known as Fourier Ptychographic Microscopy (FPM) [15, 16] showed that the limited space-bandwidth product (SBP) of a magnificationbased optical scheme can be overcome by appropriately collecting variably-illuminated images, computationally correcting aberrations, and computationally restitching the data together to create high SBP images. This current study explores another strategy for high SBP microscopy imaging
The prototype setup of the 0.5 gigapixel microscopy imaging system Driven by the recent trend of small pixel size of the image sensor (0.7 μm pixel size was reported in Ref [17].), significant efforts have been put into the design of consumer and industry camera lens to match this diffraction-limited pixel size
Summary
The conventional microscope architecture generally consists of a microscope objective for light collection from a sample slide, intermediate relay optics, and paired or single eyepieces that project a magnified image of the sample into the eyes. This paper demonstrates an optical magnification microscopy solution that challenges these assumptions The configuration of this imaging system is based on two off-the-shelf items: a closed-circuit-television (CCTV) lens and a low-cost consumer flatbed scanner. We show that such a system is capable of capturing a 0.5-gigapixel pixel image with a FOV of 75 mm and a resolution of 0.78 μm. 2. The prototype setup of the 0.5 gigapixel microscopy imaging system Driven by the recent trend of small pixel size of the image sensor (0.7 μm pixel size was reported in Ref [17].), significant efforts have been put into the design of consumer and industry camera lens to match this diffraction-limited pixel size. The captured image contained 26400 pixels × 20400 pixels, and the setup produced a 0.5 gigapixel pixel image of the sample
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