038 Single-cell sequencing of freshly isolated cells from lesional and peri-lesional skin to explore cellular origins of IL-17 isoforms in psoriasis

Abstract

Psoriasis is the most common chronic inflammatory skin disease in adults, characterized by immune cell infiltration, expansion of disease-associated lymphocytes, and epidermal hyperplasia. Increasing evidence highlights the central role of interleukin (IL)-17A and IL-17F in its pathobiology. IL-17A and IL-17F, known to be produced by activated T helper 17 cells, can form homodimers or heterodimers to drive aberrant keratinocyte biology. We applied single-cell RNA sequencing to characterize the immune infiltrate of psoriatic skin and further understand cellular sources of IL-17A and IL-17F.