0327 : Impact of a high fat diet on plasma and heart biochemical parameters of prepubertal male and female rabbits

Abstract

The biochemical cardiac and plasmatic damages induced by high-fat diet were examined in male and female rabbit and compared to the control models. Experiments were carried on 24 pre-pubertal New Zealand white rabbits, 12 males and 12 females, randomized into 4 groups (for each, n=6), 2 control groups, male (MC) and female (FC) fed a normal fat diet, and 2 High Fat Diet groups (MHFD, FHFD). HFD contained 1g of peanut butter +0.5g of animal fat. Body and heart weights were compared between the groups. Glucose, total proteins, uric acid, TG, cholesterol, phosphorus and phospholipids were examined by enzymatic methods in the plasma. The total lipids extracted from heart (Folch, 1957) were analyzed by TLC. Results obtained with HFD are compared with those of standard diet. At the end of experiment, the results showed a modest raise of body weight among MHFD vs MC, but very significant among FHFD vs FC (p<0,0001), also a significant increase of heart weight among FHFD vs MHFD, and a small raise of glycemia among MHFD vs FHFD (p<0,05).With HFD, the levels of proteins, uric acid and HDL-C were similar between female and male. The cholesterol showed a small raise in both MHFD and FHFD vs controls (p<0,05). LDL-C increased very significantly among MHFD vs MC (p<0,0001) and among FHFD vs FC(p<0,0001).There was a small raise of phospholipids in both FHFD and MHFD (p<0,05), also a small raise of TG among FHFD vs MHFD(p<0,05).In the heart, there was a small raise of total lipids for both MHFD and FHFD vs controls (p<0,05), and a small raise of phosphorus among MHFD vs FHFD, also a little raise of proteins among FHFD vs MHFD (23,85±2,61 mg/g vs 19,98±0,46 mg/g of heart).The TLC showed an increase of lipid levels among MHFD vs MC, also among FHFD vs FC. Diet supplemented with peanut butter and animal fat is responsible for major metabolic alterations in the heart and plasma of pre-pubertal male and female rabbits. The author hereby declares no conflict of interest