0281: Mitral stenosis: implication of metalloproteinase remodeling and calcification

Abstract

mitral stenosis is characterized by pathological remodelling of valvular tissue but the molecular effectors involved in these processes are not well known. The role of matrix metalloproteinase MMP-9, MMP-3, and tissue inhibitor of matrix metalloproteinase (TIMP)-1 and TIMP-2 are investigated here. Subjects and methods: 235 patients with mitral stenosis and 150 healthy control subjects were recruited. MMP-9, MMP-3, TIMP-1 and TIMP-2 levels in plasma were measured using an ELISA assay. The plasma concentrations of MMP-3, TIMP-1 were significantly lower in patients compared with control group. MMP-9 rate is significantly increased in patients with mitral stenosis. In men, a negative correlation was observed between calcification degree and rate of MMP-9 (r=–0.484, p<0.001) and positive with MMP-3 (r=0.588, p<0.001). In women, positive correlation was found between MMP-9 and mitral area (r=0.387, p=0.002) and also between MMP3 and calcification degree (r=0.603, p<0.001). This study demonstrates the involvement of the MMP/TIMP system in ECM remodelling of stenosis mitral. We have shown a difference in level of MMP- 9 between the mitral stenosis and the control. MMP9 was involved in calcification of the mitrale valve.