Abstract
Analysis of T cell proliferative responses to antigenic or mitogenic stimuli are based on e.g. the incorporation of tritiated thymidine or BrdU, CFSE labeling and the detection of secreted cytokines by ELISA or ELISPOT assay. There are inherent drawbacks of these methods rather long ex vivo expansion, stimulation protocols or the inability to distinguish specific cell populations and the assumption of importance of certain cytokines. Rapid identification and quantification of low- frequent autoreactive T cells, however, presents a main goal in autoimmune diseases such as pemphigus vulgaris (PV).
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