021 Potential role of 5-HT serotonin system in the rat spinal cord of premature ejaculation with category III prostatitis

Abstract

To explore the mechanism of premature ejaculation in rat with category III prostatitis, or chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS). We establish rat model of experimental autoimmune prostatitis (EAP) by subcutaneous multiple point's injection of male prostate gland extract emulsified in an equal volume of Freund’s adjuvant at the 0 and 21th day. The behavioral change and the sexual behavior was investigated. Then, We took out the hippocampus tissue, T13-L2 and L5- S2 spinal cord tissue after perfusion, and detected the levels of 5-HT, 5-HT1A receptor, 5-HT2C receptor and SERT in these tissues by immunohistochemical staining. The levels of 5-HT, 5-HT1A receptor, 5-HT2C receptor and SERT in the remaining rats were detected by Western Blot. The EAP model was built successfully. Serum IL- 1β and TNF-α± levels in the model were significantly higher than control (p<0.01). In the copulatory test, compared with normal control group, mount frequency (MF), intromission frequency (IF) and ejaculation frequency (EF) in the model group were significantly decreased (p <0.05); mount latency (ML), intromission latency (IL) and postejaculatory interval (PEI) in the model group were significantly shortened(p <0.05); ejaculation latency (EL) in the model group was significantly significantly prolonged (p <0.05). In hippocampus tissue, 5-HT levels in the model were significantly lower than normal (p<0.05), whereas 5-HT1A, 5-HT2C and SERT levels in the model were significantly higher than normal (p<0.05). In T13-L2 spinal cord tissue, 5-HT levels in the model were significantly lower (p<0.05), 5-HT1A receptor levels in the model were significantly increased (p<0.05). In L5-S2 spinal cord tissues, 5-HT levels in the model were significantly lower (p<0.05), while the 5-HT1A, 5-HT2C and SERT levels in the model increased slightly, but the differences in 5-HT1A, 5-HT2C and SERT levels between the two group were statistically significant (p<0.05).