019 Single cell level protein analysis of autoantigen-reactive B cells in systemic sclerosis and the murine model

Abstract

Since the antigen affinity of B cells varies from cell to cell, functional analysis in each B cell has been difficult from technical aspects. Especially in autoimmune diseases, promising pathogenic B cells have not been adequately studied to date because of the rarity. Here, we analyzed the single-cell function of autoantigen-reactive B cells in systemic sclerosis (SSc). Topo I-reactive B cells were extracted from peripheral blood of SSc patients. mRNA expression and protein expression analysis, co-culture analysis with T cells in micro-space, and cytokine and antigen binding capacity analysis were performed.