016 Uspio-enhanced CMR comprehensive methodological investigation and application in acute MI

Abstract

Introduction Quantification of active myocardial inflammation may improve diagnosis, guide management and provide trial end-points for novel therapies. Ultrasmall particles of iron oxide (USPIO) are phagocytosed by activated leukocytes and USPIO-enhanced CMR is increasingly used to assess tissue inflammation. We aimed to; 1. Compare T2* imaging with T1 mapping, which is proposed as an alternative for ‘native’ cardiac iron measurement; 2. Determine whether imaging at a single time point post-USPIO is sufficient to detect active accumulation in tissue; 3. Determine whether USPIO signal from infarct and remote zones in acute myocardial infarction (MI) reflects active myocardial accumulation or passive ‘wash-through’ in oedematous myocardium. Methods Four healthy volunteers and six patients with acute MI underwent 1.5T CMR, including T1 and T2* mapping, before and at multiple time points following 4 mg/kg ferumoxytol. Results Normalised T2* of spleen, an organ with high active leukocyte activity, dropped post-USPIO and remained low over the study period (Figure 1), with no correlation seen between spleen T2* and blood T1 (rho=−0.43, p=0.875). In comparison, T1 recovery in spleen correlated strongly with T1 recovery in blood (rho=0.924, p In MI, absolute T2* values dropped and remained significantly lower in infarcted (15 vs 27ms, p Conclusions 0T2* is sensitive to active tissue accumulation of USPIO, likely because T2* reflects field gradients, such as those generated by compartmentalised (phagocytosed) USPIO. T1, which is due to short range dipolar interactions that reduce as USPIOs wash-out, simply tracks passive wash-through. T1 is therefore less suitable for detecting active leukocytes. Measuring T2* at a single time point post-USPIO is insufficient to determine tissue accumulation from passive wash-through. USPIO signal from infarct and remote zones in acute MI appears to genuinely reflect active myocardial accumulation, presumably due to phagocytosis by activated leukocytes.