Abstract
Background:Tissue-resident memory T (TRM) cells reside in peripheral tissues, including the skin. In humans, TRM cells exist in both the epidermis and dermis, but their composition, phenotype, and function are not fully elucidated. To analyze T cells in both the epidermis and dermis, the skin needs to be incubated with Dispase II to separate the two layers. The next step varies among researchers; the subsequent enzymatic digestion of epidermis and dermis is popular, whereas the spontaneous migration method can also be done.
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