Abstract
Ultra-fast cooling induced by total liquid ventilation (TLV) has been shown to be potently neuro and cardioprotective after shockable cardiac arrest and/or acute myocardial infarction . In this study we examined a possible underlying mechanism of this protection, in particular reduced free radicals production. Thirty six rabbits subjected to asphyxia cardiac arrest were divided into three groups: normothermic life support (Control group, n=12) or hypothermia induced by either i.v. cold saline (CONV group; n=12) or by TLV (TLV group, n=12). Using electronic paramagnetic resonance spectroscopy (EPR) and 1-hydroxy-3-methoxycarbonyl-2,2,5,5-tetramethylpyrrolidine hydrochloride (CMH) as spin probe, we observed a decrease in the production of free radicals in various organs including the heart and the cerebral cortex in TLV and CONV groups as compared to Control, however the effect of TLV was significantly more marked. These results were associated with a significant improvement of the neurological status and an increase in the survival rate as compared to Control and CONV groups. Therapeutic hypothermia induced by TLV could be a promising approach to improve organ preservation before irreversible alteration by free radicals.
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