저항운동이 ZDF쥐의 대동맥에서 ER stress 억제에 관한 연구

Abstract

OBJECTIVES: The purpose of this study was to investigate the effects of resistance exercise training (RET) on Endoplasmic reticulum (ER) stress in aorta of diabetes model rats.BR MATERNAL/METHODS: All experimental rats (age. 8 weeks old) were separated into 3 groups after 1 week adaptation and namely zucker lean control (ZLC-C, n=6) and diabetic control (ZDF-C, n=6), and diabetic resistance exercise (ZDF-EX, n=6) groups. The experimental rats were RET began a week later. RET was performed by 10 repetitions of climbing a leader 5 days per week for 8 weeks. Serum glucose (GL) and body weight (BW) were measured on the 1st and 8 weeks of experimental period. All rat aorta samples were harvested for biochemical analysis and also performed ER stress protein (PERK, IRE-1 and ATF-6) expression by Western blot.BR RESULTS: The RET significantly inhibited BW and levels of serum GL compared with untreated ZDF-C. ZDF-EX group was markedly decreased PERK, IRE-1 and ATF-6 expression compared to ZDF-C group. In addition, this group had higher eNOS expression in the aorta.BR CONCLUSION: These results suggest that RET suppressed the activation of ER stress repose following diabetes induced ER stress, and it has beneficial effects in terms of protecting against ER stress by controlling PERK, IRE-1 and ATF-6 expression, as well as eNOS in the aorta of Zuker diabetic rats. Thus, therapeutic strategies that eNOS might be a potential mediator for the prevention effect of RET on the ER stress progress in type 2 diabetic aorta.