Стимуляция ангиогенеза матрицами из поликапролактона, содержащими VEGF

Abstract

Purpose. To evaluate the local angiogenesis stimulation by polycaprolactone (PCL) scaffolds with VEGF in vivo. Materials and methods. PCL scaffolds were fabricated using electrospinning method. For the encapsulation of VEGF into PCL scaffold a two phase electrospinning was used. The scaffolds structure was examined by scanning electron microscopy. Growth factor release dynamic was assessed by ELISA. For evaluation of biological properties, the PCL and PCL+VEGF scaffolds were implanted in Wistar rat abdominal wall (n=60) for 2, 3 and 4 monthsT. he explanted samples were examined by histological and immunoishtochemical analyses. Results. The study showed that PCL fiber diameters in the scaffolds have changed after VEGF encapsulation. Moreover, long-term controlled release of growth factor was demonstrated. In addition, we have also shown the preservation of PCL+VEGF scaffolds biological activity; this was evidenced by increase of the number of capillaries on scaffolds with VEGF compared to control samples after implantation in rats. Conclusion. This study showed that the PCL scaffold with VEGF has pro-angiogenic potential in comparison with pure scaffolds and can potentially be used for the tissue engineering in vivo.