Abstract

In a preceding report3), it was shown that the addition of the viscera-extract of Pecten laqueatus at a 1.7 per cent dry weight basis gave a remarkable growth response of chicks and that APF in the viscera-extract was adsorbed on the activated charcoal from a slightly acidic solution. The present paper deals with a study on the effectiveness of an autolyzed viscera-extract of Atrina Pectinata japonica to the chick growth and the egg production, and further on the fractionation of the growth promoting principle. The results can be summarized as follows. 1. The addition to 100g. of all-vegetable protein ration of a 2.8g. portion of the dried autolyzed viscera and a 0.3g. portion of an activated charcoal adsorbate which is equivalent to 2.8g. of the dried autolyzate, resulted in a slight growth promotion on 35-day old chicks during six-week feeding as contrasted to a negative result obtained on addition of 1.35g. of a non-adsorbed fraction (Table 1). 2. The weight of anterior hypophysis and testis, and the length of humerus and femur increased in a linear proportion to the growth while the weight of thyroid gland inversely pro-portional (Table 2). 3. One-year old hens fed autolyzed viscera at a 2% dry weight basis for 100 days showed an egg production inferior to the control group. This is apparently due to a delay of starting egg production in the experimental group, because when compared as to the egg production of its highest production period (Feb. 7-March 30), a superior egg production in number as well as weight was observed in the experimental group (Table 3). 4. In the case of 5-month old hens fed an all-vegetable protein ration for 3 months prior to the experiment, the supplementation of the autolyzate at a 2% dry weight basis gave a remarka-ble response in egg production (Table 4).

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