台灣地區 Umbelipsis 屬真菌之分類研究

Abstract

The main object of this study is the genus Umbelopsis ( Umbelopsidaceae , Mucorales,Mucoromycotina, Fungi) from Taiwan. They are ubiquitously saprotrophic fungi and can be isolated from soil and fallen leaves. The distinctive morphological characters of Umbelopsis are sporangiophore with basal vesicle bearing branches, red to brown colony color on PDA, variable spore shape and apical single sporangium. This study has collected 858 soil samples. Based on morphological characters, five species which are Umbelopsis angularis, U. dimorpha, U. isabellina, U. rammaniana, U. angularis, U. dimorpha and U. vinacea were identified. Three of these species are new records to Taiwan. Molecular biotechnology including polymerase chain reaction (PCR), and sequencing were used to obtain the sequence of ribosomal DNA LSU-D1/D2 domain and ITS sequence (ITS1, 5.8S rDNA, ITS2). The Umbelopsis caldes is divided into two well-supported subclades, the first subcalde,contains Umbelopsis dimorpha, U. isabellina and U. vinacea. The second caldes, contains Umbelopsis angularis and U. rammaniana. U. isabellina, U. rammaniana and U. vinacea can be separated individually. Umbelopsis dimorpha and U.versiformis are closely posifioned on the tree which have of single-spored sporania. Taxonomic groups generating from molecular analysis of these gene regions of rDNA are consistent with the groups based on morphological characters. Consequently, based on combined data obtaining from the phylogenetic analysis and morphological characters, a key of the genus Umbelopsis in Taiwan was constructed.