α-Tocopherol을 함유한 Nanostructured Lipid Carriers의 특성과 안정화 효과

Abstract

Loading of hydrophobic α-tocopherol into nanostructured lipid carrier (NLC) was performed for improving its oxidative stability. First, various NLCs with different constituents and mixing ratios were prepared and their characteristics were investigated. While the stable NLCs were made when cetyl palmitate (CP) or glyceryl monosterate (GMS) was used as a solid lipid, the phase separation occurred in the NLCs consisting of stearic acid. Particle sizes of the NLCs were several hundreds of nanometers and the size decreased with increasing the ratio of solvent to lipid. It was examined from DSC thermogram and anisotropy test that the degree of crystallinity of the lipid phase decreased and the lipid matrix became less ordered when octyldodecanol, a long chain fatty alcohol, was added into the solid lipid. The oxidative stability of α -tocopherol in NLC was remarkably improved compared to that in solution or emulsion under high temperature (45 °C) and UV radiation, which was verified through DPPH test and peroxide value measurement.