Abstract
&gamma;&delta;-T-Cell Receptor (TCR) lymphocytes were detected in mammary gland lavages collected from 10 clinically healthy virgin heifers before and after intramammary stimulation with synthetic muramyl dipeptide analogue. Using two-colour flow cytometry, CD2<sup>+</sup> and CD2<sup>&ndash;</sup> subsets of &gamma;&delta;-TCR lymphocytes were analyzed. CD2<sup>+</sup> &gamma;&delta;-TCR lymphocytes markedly prevailed over CD2<sup>&ndash;</sup> cells in intact mammary gland: 88.9 &plusmn; 4.9% of &gamma;&delta;-TCR lymphocytes were CD2<sup>+</sup>. After stimulation, neutrophils and &gamma;&delta;-TCR lymphocytes were recruited into the mammary gland. Among &gamma;&delta;-TCR lymphocytes, CD2<sup>&ndash;</sup> cells were mainly responsible for their expansion. After stimulation, 60.8 &plusmn; 13.4% of &gamma;&delta;-TCR lymphocytes were CD2<sup>+</sup> (P &lt; 0.01 when compared with mammary gland lavages before stimulation). It follows from the present study that the cells seem to be involved in the first phase of a response to an infection affecting mammary gland.
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