β-Sitosterol: Supercritical Carbon Dioxide Extraction from Sea Buckthorn (Hippophae rhamnoides L.) Seeds

Various seeds, including sea buckthorn (Hippophae rhamnoides L.) seeds, are sources of different bioactive compounds. They can show anti-inflammatory, hypoglycemic, anti-hyperlipidemic, antibacterial, antioxidant, or other biological properties in in vitro and in vivo models. Our preliminary in vitro results have demonstrated that the extracts from raw (no thermal processing) and roasted (thermally processed) sea buckthorn seeds have antioxidant potential and anticoagulant activity. However, it was unclear which compounds were responsible for these properties. Therefore, in continuation of our previous study, the extracts were fractionated by C18 chromatography. Phytochemical analysis of three fractions (a, b, and c) from raw sea buckthorn seeds and four fractions (d, e, f, and g) from roasted sea buckthorn seeds were performed. Several in vitro assays were also conducted to determine the antioxidant and procoagulant/anticoagulant potential of the fractions and two of their major constituents—isorhamnetin 3-O-β-glucoside7-O-α-rhamnoside and serotonin. LC–MS analyses showed that serotonin is the dominant constituent of fractions c and f, which was tentatively identified on the basis of its HRMS and UV spectra. Moreover, fractions c and f, as well as b and e, contained different B-type proanthocyanidins. Fractions b and e consisted mainly of numerous glycosides of kaempferol, quercetin, and isorhamnetin. The results of oxidative stress assays (measurements of protein carbonylation, lipid peroxidation, and thiol groups oxidation) showed that out of all the tested fractions, fraction g (isolated from roasted seeds and containing mainly dihexoses, and serotonin) demonstrated the strongest antioxidant properties.

Antioxidant-rich fractions were extracted from sea buckthorn (Hippophae rhamnoides) seeds using various solvents, including acetone, methanol, ethanol, ethyl acetate, water and mixtures of different solvents, and analyzed for their phenolic components. The antioxidant activity of the extracts was evaluated using a copper-catalyzed lecithin liposome oxidation assay. At 250 μg/mL concentration, the sea buckthorn seed extracts inhibited formation of conjugated diene hydroperoxides and thiobarbituric acid reactive substance (TBARS) by 0-90% and 0-88.6%, respectively. 70% acetone extract showed the highest antioxidant activity. The extract also showed good reducing power and DPPH radical scavenging capacity. Proanthocyanidins were the predominating polyphenols while hydroxycinnamates and flavonols represent minor components in all extracts. Positive correlations were found between proanthocyanidin content and antioxidant activities of sea buckthorn seed extracts.

Sea buckthorn (Hippophae rhamnoides L.) is a small tree, valued for its medicinal properties throughout the ages. Sea buckthorn berries and leaves are a known source of phytochemicals and have been used in the treatment of inflammation, oedema, hypertension, ulcers, and wounds in folk medicine. Sea buckthorn seeds are natural dietary sources of various bioactive compounds as well, but the number of studies on their content and biological properties is still insufficient. For the first time, we examined the phytochemical content and biological activity of sea buckthorn seeds in vitro. We have studied the effect of two extracts—from regular (no thermal processing) and roasted (thermally processed) sea buckthorn seeds—on the levels of oxidative stress induced by H2O2/Fe2+ in plasma, coagulation times, and white thrombus formation (measured by Total Thrombus-formation Analysis System—T-TAS). We observed that sea buckthorn seeds contain diverse flavonoids, mostly glycosides of isorhamnetin, kaempferol, and quercetin, as well as smaller amounts of proanthocyanidins and catechin, triterpenoid saponins, and a number of unidentified polar and hydrophobic compounds. Both extracts inhibited lipid peroxidation and protein carbonylation, but only the extract from roasted seeds decreased oxidation of thiol groups in plasma treated with H2O2/Fe2+. They did not alter coagulation times, but the extract from roasted seeds at the highest concentration (50 µg/mL) prolonged the time needed for white thrombus formation. The results indicate that sea buckthorn seeds have antioxidant activity that is not impaired by thermal processing and possess anticoagulant potential, but more research is needed in order to ascertain which compounds are responsible for these effects, especially in in vivo models.

Background: Given the pivotal role of diet in cardiovascular diseases (CVDs), there is a growing demand for new sources of bioactive phytochemicals that can contribute to CVD prevention and treatment. Previous research has unveiled the cardioprotective properties of several parts of sea buckthorn (Hippophae rhamnoides L.). For example, various fractions isolated from raw and roasted sea buckthorn seeds showed antioxidant properties in vitro. In addition, the serotonin-rich fraction obtained from roasted seed extract had the strongest antioxidant activity. However, it was unclear which chemical constituents contribute to the anti-platelet potential of sea buckthorn seeds. Methods: The anti-platelet activity of two fractions (fraction b and fraction c) from raw sea buckthorn seed extract, two fractions (fraction d and fraction g) from roasted sea buckthorn seed extract, and two chemical compounds—isorhamnetin 3-O-β-glucoside-7-O-α-rhamnoside (a major component of fraction b), and serotonin (5-HT, 5-hydroxytryptamine), present in fraction c was estimated in several in vitro assays. Results: Isorhamnetin 3-O-β-glucoside-7-O-α-rhamnoside significantly inhibited platelet activation. It lowered the exposition of the active form of GPIIb/IIIa on the surface of 20 μM ADP-stimulated platelets by about 26%. It also inhibited the exposition of P-selectin on the surface of 10 and 20 μM ADP-stimulated platelets. In addition, isorhamnetin 3-O-β-glucoside-7-O-α-rhamnoside (at 50 µg/mL) significantly prolonged the time of thrombus formation. The results also indicate that fractions d and g (from roasted seeds) are more effective anti-adhesive factors than fractions from raw sea buckthorn seeds. Conclusions: It can be suggested that sea buckthorn seeds can serve as a new source of anti-platelet compounds (especially derivatives of isorhamnetin) beneficial in CVD prevention and treatment; however, in vivo research is needed to clarify their mechanism of action, physiologically relevant concentrations, and therapeutic potential.

Hippophae rhamnoides, also named as Sea Buckthorn(SB) is a spiny bush with long and narrow leaves, and orange yellow berries. It is native to Europe and mainly to Asia. The leaves, fruits and the seeds of sea bucHippophae rhamnoides, also named as Sea Buckthorn(SB) is a spiny bush with long and narrow leaves, and orange yellow berries. It is native to Europe and mainly to Asia. The leaves, fruits and the seeds of sea buckthorn contain many bioactive compounds. These compounds are various natural antioxidants such as ascorbic acid, tocopherols, carotenoids, flavonoids, whereas besides them all we can find macronutrients such as proteins, lipids (mainly unsaturated fatty acids and phytosterols), vitamins (especially vitamin C), minerals, small amounts of sugars and organic acids . The present review focuses on the functionality of SB lipids and refers to those studies that suggest the sea buckthorn could have various beneficial effects on cardiovascular health, insulin and glucose response, renal health, hepatic health, Gastric health, epidermis health, cancer, ophthalmologic health and oxidative stress and toxicity.kthorn contain many bioactive compounds. These compounds are various natural antioxidants such as ascorbic acid, tocopherols, carotenoids, flavonoids, whereas besides them all we can find macronutrients such as proteins, lipids (mainly unsaturated fatty acids and phytosterols), vitamins (especially vitamin C), minerals, small amounts of sugars and organic acids . The present review focuses on the functionality of SB lipids and refers to those studies that suggest the sea buckthorn could have various beneficial effects on cardiovascular health, insulin and glucose response, renal health, hepatic health, Gastric health, epidermis health, cancer, ophthalmologic health and oxidative stress and toxicity.

Sea buckthorn (Hippophae rhamnoides L.) is an oil crop with health benefits. Its fruits are rich in unsaturated fatty acids (FAs); however, the FA composition of the seeds and pulp differs significantly. To evaluate the expression levels of gene families that play a major role in FA biosynthesis, the transcriptomes of seeds and pulp at four fruit development stages were sequenced for five sea buckthorn varieties with diverse characteristics: Elizaveta, Inya, KP-686, Panteleevskaya, and Triumf. The results revealed that FAD3 (07426) and FAD3 (05528) are likely key genes for linolenic acid synthesis in seeds, while FAD2 (21624) is likely the main contributor to linoleic acid synthesis in both seeds and pulp. SAD (18830) primarily contributes to oleic acid synthesis in seeds, while SAD (18830) and SAD (26748) contribute to its synthesis in pulp. FATA (14745) and FATA (14109) are also implicated in FA synthesis in sea buckthorn fruits. Changes in the content of the main FAs in seeds and pulp correlated with the expression levels of the corresponding genes. KP-686 and Triumf differed the most from other varieties. These results are important for analyzing tissue-specific gene expression in seeds and pulp of sea buckthorn fruits, and they are promising for developing sea buckthorn varieties with improved oil composition.

Sea buckthorn fruits (Hippophae rhamnoides L.) are valuable raw materials for the food and pharmaceutical industries due to the significant amount of oil in the fruit pulp and seeds, characterized by a unique combination of components. The primary substance of the oil is a complex of fatty acids, the composition of which has been well-studied for most genotypes. Still, studies have not been carried out in the group of sea buckthorn varieties of Altai selection. This study aimed to compare the fatty acid composition of the pulp of fruits and seeds of sea buckthorn selection by the Research Institute of Horticulture of Siberia, named after M.A. Lisavenko. The object of the study was the fruits of 13 sea buckthorn varieties belonging to the subspecies Hippophae rhamnoides ssp. mongolica, collected in 2014–2015. in the phase of full ripeness at the experimental sites of the Research Institute of Horticulture of Siberia, named after M.A. Lisavenko, is located in the forest-steppe of the Altai Territory. Determination of fatty acid composition was carried out in fruit pulp and sea buckthorn seeds using gas chromatography. Six fatty acids were identified in the fruit pulp of sea buckthorn, differing significantly in percentage. The predominant ones are palmitic (C16:0) and palmitoleic acids (C16:1), which account for more than 80% of all fatty acids. The most minor amounts are stearic (C18:0) and linolenic (C18:3) acids (2.32%). Sea buckthorn seed oil is dominated by unsaturated fatty acids (more than 90%): oleic (C18:1), linoleic (C18:2) and linolenic acid, and cis-vaccenic acid (C18:1-n7) are present. The largest share on average for varieties is linoleic acid (40.93%). The content of palmitoleic acid in the seed oil was 0.29%, palmitic acid – 6.95%, and stearic acid – 2.04% of the total acids.

Essential oils extraction from Anoectochilus roxburghii using supercritical carbon dioxide and their antioxidant activity

Sea buckthorn (Hippophae rhamnoides L.) is a tree or shrub with small, orange berries. Sea buckthorn seeds have shown many properties beneficial to human health, including antioxidant, anti-hypertensive, anti-hyperlipidemic, and retinoprotective activities. Seeds, as a component of food, are often exposed to high temperatures, which can increase or decrease their biological activity. In our previous study, we showed that both raw and roasted sea buckthorn seeds had significant antioxidant activity, which was measured in human plasma in vitro. In this paper, we evaluated the effect of extracts from raw and roasted sea buckthorn seeds on several parameters of hemostasis in vitro, including thrombus formation in full blood (measured by the Total Thrombus formation Analysis System-T-TAS), blood platelet activation (based on the exposition of P-selectin, the active form of GPIIb/IIIa on their surface and platelet-derived microparticles formation), aggregation (measured with impedance aggregometry), adhesion to fibrinogen and collagen, arachidonic acid metabolism in washed platelets stimulated by thrombin, and COX-1 activity. We also measured the levels of free 8-isoprostane in plasma and the total non-enzymatic antioxidant status of plasma. The extract from roasted seeds (50 µg/mL) significantly prolonged the time of occlusion measured by T-TAS-the AUC10 (area under the curve) value was decreased by approximately 18%. Both extracts decreased the exposition of the active form of GPIIb/IIIa on the surface of platelets activated with 10 μM ADP (by 38.4-62.2%) and 20 μM ADP (by 39.7-51.3%). Moreover, the extract from raw seeds decreased the exposition of P-selectin on the surface of platelets stimulated with 20 μM ADP (by 31.2-34.9%). The adhesion of thrombin-stimulated platelets to fibrinogen and collagen was inhibited only by the extract from roasted sea buckthorn seeds (by 20-30%). Moreover, the extract from raw seeds inhibited the level of TBARS (thiobarbituric acid-reactive substances, an indicator of enzymatic peroxidation of arachidonic acid) in washed platelets stimulated with thrombin; the activity of COX-1 was inhibited by both extracts, although the effect of the extract from raw seeds was stronger. These results indicate that sea buckthorn seeds have anti-platelet activity that is not decreased by thermal processing, but more research is needed to determine which exact chemical compounds and mechanisms are responsible for this phenomenon.

The sea buckthorn plant (Hippophae rhamnoides L.) helps treat a wide range of short- and long-term illnesses. Its therapeutic and pharmacological properties have been extensively studied through the use of many in vitro and in vivo models. Undoubtedly, the future offers much potential for SBT bio-actives. There are 18 distinct kinds of essential amino acids and 24 critical minerals in seabuckthorn juice. In addition to a healthy balance of omega-3 to omega-6 fatty acids, the oil extracted from seabuckthorn seeds is particularly rich in oleic acid. In addition to protecting the skin from harmful UV rays, the oil helps keep the skin healthy. Traditional uses of the plant to heal a wide range of diseases have been confirmed and built upon by recent scientific research. Those in fields as diverse as biotechnology, nutraceuticals, pharmaceuticals, cosmetics, and the environment may all learn something from the seabuckthorn shrub because of its unique and valuable features. Hypertension, edoema, ulcers are just some of the ailments that this plant's berries, seeds, and leaves are used to cure in its traditional folk medicine form. other beneficial chemicals have all been identified via phytochemical analysis. Our research showed that SBL has a lot of valuable nutrients, including protein and minerals.The optimal conditions for organic acid profiling in SB berries were initially established using RP-HPLC-DAD analysis. The plant's beneficial and therapeutic characteristics have been studied intensively for decades. The presence of bioactive substances such as triterpenoids, saponins, and ellagitannins gives Hippophae rhamnoides its therapeutic potential.

Lipophilic extracts isolated from European cranberry bush (Viburnum opulus) and sea buckthorn (Hippophae rhamnoides) berry pomace by supercritical CO2 – Promising bioactive ingredients for foods and nutraceuticals

Sea buckthorn (Hippophae rhamnoides L.) is an important plant with homology of medicine and food. It has rich nutritional and medicinal properties. It is used as a traditional Chinese medicine with therapeutic functions of invigorating spleen, relieving cough, eliminating food, eliminating phlegm, dispersing blood stasis, and promoting blood circulation. This review comprehensively summarized flavonoids from sea buckthorn (Hippophae rhamnoides L.), including extraction methods (solvent extraction, ultrasound-assisted extraction, microwave-assisted extraction, enzyme-assisted extraction, and collaborative extraction), two structure types (18 flavone aglycones and 81 flavone glycosides), detection methods (UV, HPLC, and NMR), bioactivities (antiviral, anti-inflammatory, hepatoprotective, weight-reducing, and hypoglycemic activities), and physiological metabolisms (most of flavonoids are converted into small molecule monophenolic acids through intestinal microbial catabolism). It will supply an important theoretical basis and valuable reference for researching and exploiting sea buckthorn (Hippophae rhamnoides L.) in the future. Practical Applications. Sea buckthorn (Hippophae rhamnoides L.) is an edible and medical plant with many functional properties. A comprehensive review on extraction, structure, detection, bioactivity, and metabolism of flavonoids from sea buckthorn (Hippophae rhamnoides L.) was made in this paper. This review will provide an important foundation for further studies of sea buckthorn (Hippophae rhamnoides L.) focusing on its development and utilization.

Sea buckthorn (Hippophae rhamnoides L.) seed, which has very high nutraceutical, cosmetoceutical and therapeutic properties, has been widely used to treat diseases in Tibetan and Mongolian traditional medicines. In this study, we measured the antioxidant activities of the solvent-dependent SBS (Sea buckthorn seed) extracts using the DPPH and Folin-Ciocalteu assays and we investigated the efficacy of SBS in protecting skin against UVB-induced damage using cultured human dermal fibroblasts. In addition, the inhibitory effects of SBS on UVB-induced skin photoaging were examined by determining the level of Metalloproteinase (MMP)-1 and procollagen after UVB-irradiation. The antioxidant capacity of the SBSE (alcoholic) extract was significantly higher than the SBSH (hexane) and SBSW (water) extracts, as measured based on the free-radical scavenging activity and total phenolic content. SBSE was chosen as the most suitable candidate antioxidant. In human dermal fibroblasts, the cell viability of SBSE extract at doses of 2.5, 5, and 10 μg/mL was higher than the UVB-treated control. By RT-PCR and Western blot, SBSE treatment inhibited UVB-induced IL-1β expression in cultured cells. In addition, SBSE restrained UVB-induced IL-6 and COX-2 gene expression in cultured fibroblasts in a dose-dependent manner. Treatment with the SBSE extract after UVB irradiation in human dermal fibroblasts significantly reduced MMP-1 expression and increased procollagen synthesis when compared with UVB-irradiation only. In this study, SBSE was shown to increase the synthesis of procollagen, decrease the expression of MMP-1, and inhibit the production of IL-1β, IL-6, and COX-2 in UVB-irradiated human fibroblasts. These findings suggest that the SBSE extract may be a potential therapeutic agent for preventing and treating skin photoaging.

Oil and xanthorrhizol extraction from Curcuma xanthorrhiza Roxb. rhizome by supercritical carbon dioxide was optimized using Taguchi method. The factors considered were pressure, temperature, carbon dioxide flowrate and time at levels ranging between 10-25MPa, 35-60°C, 10-25g/min and 60-240min respectively. The highest oil yield (8.0%) was achieved at factor combination of 15MPa, 50°C, 20g/min and 180min whereas the highest xanthorrhizol content (128.3mg/g oil) in Curcuma xanthorrhiza oil was achieved at a factor combination of 25MPa, 50°C, 15g/min and 60min. Soxhlet extraction with n-hexane and percolation with ethanol gave oil yield of 5.88%, 11.73% and xanthorrhizol content of 42.6mg/g oil, 75.5mg/g oil, respectively. The experimental oil yield and xanthorrhizol content at optimum conditions agreed favourably with values predicted by computational process. The xanthorrizol content extracted using supercritical carbon dioxide was higher than extracted using Soxhlet extraction and percolation process.

This study employed wild sea buckthorn (Hippophae rhamnoides L.) fruits harvested in Qinghai Province as experimental material. Following compositional analysis of their flavonoids, the antibacterial efficacy and mechanistic pathways of flavonoids in sea buckthorn against Helicobacter pylori (H. pylori) were systematically examined through in vitro and animal model experiments. The results showed that the main flavonoids in sea buckthorn were rutin, quercetin-3-O-glucoside, quercetin, catechin, isorhamnetin, kaempferol-3-O-glucoside, kaempferol and epicatechin. Among them, quercetin, isorhamnetin and kaempferol had strong inhibitory activity against H. pylori. In vitro, sea buckthorn flavonoids inhibited the growth of H. pylori through multiple mechanisms: inducing morphological transformation from rod-shaped to spherical bacteria, promoting cell shrinkage and rupture, disrupting the cell membrane to cause leakage of intracellular macromolecules, increasing membrane permeability, and suppressing urease activity. Sea buckthorn flavonoids exert therapeutic effects on H. pylori-infected mice through multiple mechanisms, including the alleviation of gastric mucosal inflammation via the Nuclear Factor KappaB (NF-κB) signaling pathway, the down-regulation of gastrin-17 (GAS17) to suppress gastric acid production, and the up-regulation of epidermal growth factor (EGF) expression to promote gastric mucosal repair and modulate the composition of gastric microbiota. This study systematically elucidated the anti-H. pylori activity and antibacterial mechanisms of flavonoids derived from sea buckthorn fruits, providing a theoretical basis for the screening of natural antibacterial agents from this plant source.