생장억제제 처리가 나도풍란(Sedirea japonica) 유묘의 생장과 invertase 활성에 미치는 영향

Abstract

본 연구는 몇 가지 생장억제제를 배지에 처리하였을 때 기내 배양한 나도풍란 유묘의 생장과 그들의 invertase활성 검정을 위하여 실시하였다. 나도풍란에 식물생장억제제 인 ancymidol과 paclobutrazol을 각각 배양용 배지에 첨가했을 때, 농도가 높을수록 엽장이 감소한 반면, 엽폭은 상대적으로 증가하였고, 근장은 짧았으나 근수 및 근직경은 증가하였다 Uniconazole 0.05 mg/L에서는 잎과 뿌리생장이 대조구보다 오히려 촉진되었다. 처리한 모든 종류에 있어서 그 농도가 높아짐에 따라 잎 모양은 원형으로 변하면서 작아졌다. Invertase 활성을 알아보기 위 한 soluble acid invertase 활성과 soluble alkaline invertase활성은 잎에서는 생장억제제 농도가 낮은 처리에서 높았고, 뿌리에서는 정반대의 경향이었다. 지상부 및 지하부의 전분함량은 잎에서는 invertase 활성이 높았던 저농도에서 높았으며, 뿌리에서는 invertase 활성이 높았던 고농도에서 높았다. This experiment was conducted to identify the effect of several plant growth retardants on growth of Sedirea japonica seedlings cultured in vitro and their changes of invertase activities. When seedlings of Sedirea japonica were treated with ancymidol and paclobutrazol, as the concentrations were increased, leaf length was gradually shortened and leaf width became wider than that of control. On the other hand, root length was shorter, but the number of root and the root's diameters were greatly increased, compared with control. In 0.05mg/L uniconazole, growth of leaf and root were enhanced, compared with the control and higher concentrations of uniconazole. As concentration of each growth retardants was increased, leaf shape became round and smaller. Both soluble acid invertase activity and soluble alkaline invertase activity in leaf were decreased in higher concentrations of each growth retardant, but those of the root were contrary to those of the leaf. To confirm the estimated invertase activities, starch content of leaf was higher in low concentration treatments in each growth retardant, but in the root was contrary to content that of the leaf.