Аналіз застосування біотехнологій щодо одержання високоякісного садивного матеріалу рослин родини Salicaceae Mirb. in vitro для створення біоенергетичних плантацій

Abstract

In conditions of a sharp reduction in the stocks of traditional fuels, it is important to find new efficient and renewable carbon-neutral energy sources. One of the most promising sources of renewable energy is the biomass of woody plants, in particular the family Willow (Salicaceae Mirb.). Traditionally, Salicaceae plants are propagated by generative and vegetative methods. Microclonal propagation, in contrast to traditional methods of reproduction, allows to obtain genetically homogeneous healthy planting material throughout the year. A significant number of biotechnological publications focus on the development of the optimal protocol for the reproduction of plants of the family Salicaceae, the study of the morphogenetic potential of tissues and optimizing their growth. However, the authors note the individually determined regenerative ability of plant material in vitro, which depends on a number of factors. The purpose of the study is to analyze the results of biotechnological research on the effectiveness (possibility) of obtaining high-quality planting material of plants of the family Salicaceae by tissue in vitro. For this purpose, we used the results of biotechnological studies of plant tissues of the family Salicaceae in vitro by foreign and domestic authors published in professional journals during 2010−2020. Research methods − analysis, comparison, synthesis, generalization. The analysis revealed that the sterilization regime of Salicaceae plant material depends on the type of explant, phenological phase and age of the donor plant. Step-by-step sterilization using mercury chloride, sodium hypochlorite and silver nitrate effectively neutralizes the exogenous biota of woody explants. The stage of active vegetation is the optimal period of isolation of explants. To obtain virus-free regenerating plants, it is advisable to use apical meristems, callus tissue − leaf blades, active regeneration − microshoots. Stable regeneration system, its type, multiplication factor of Salicaceae plants are genetically determined. For the introduction of plants in vitro, active proliferation, rooting, microclonal propagation, it is advisable to use nutrient media according to WPM (McCown & Lloyd, 1981) and MS (Murashige & Skoog, 1962). For regeneration of plants by direct morphogenesis and activation of growth of existing meristems of an explant to apply the environment with cytokinins (BAP (6-benzylaminopurine), kinetin or 2-isopentenyladenine (2-ip), for rooting − with auxins NAA (α-naphthylacetic), IBA (3-indolylbutyric acid) and IAA (β-indolyl-3-acetic acid). Further research is aimed at optimizing the propagation protocols of Salicaceae plants in vitro.