마늘성분 SAC 및 Hydrogen Peroxide에 의한 줄기세포의 유전자 발현 윤곽

Abstract

Though hydrogen peroxide (<TEX>$H_2O_2$</TEX>) causes a deleterious effect to cells with its reactive oxygen species resulting in cell death, S-allyl cysteine (SAC, a bioactive organosulfur compound of aged garlic extract) has been known to have a cytoprotective effect. Few reported profiles of gene expression of <TEX>$H_2O_2$</TEX> and SAC treated human cord blood derived mesenchymal stem cells (MSC). This study revealed changes in the profile of twenty-one genes grouped by oxidative stress, antioxidant, cell death, anti-apoptosis and anti-aging by quantitative real time PCR. A concentration of <TEX>$100{\mu}M$</TEX> of SAC or <TEX>$50{\mu}M$</TEX> of <TEX>$H_2O_2$</TEX> was applied to MSC which show moderate growth and apoptosis pattern. <TEX>$H_2O_2$</TEX> treatment enhanced expression of eleven genes out of twenty-one genes compared with that of control group, on the contrary SAC suppressed expression of eighteen genes out of twenty-one genes except C ros oncogene. SAC decreased expression of oxidative stress genes such as SOD1, CAT and GPX. These results seemed consistent with reports which elucidated over-expression of NF-<TEX>${\kappa}$</TEX>B by <TEX>$H_2O_2$</TEX>, and suppression of it by SAC. This study will confer basic information for further experiments regarding the effects of SAC on gene levels.