Частота полиморфизма rs776746 в гене CYP3A5 у женщин с неразвивающейся беременностью

Abstract

Purpose. The purpose of this study was to examine frequency of genetic polymorphism of CYP3A5*3 (rs776746 A>T in women with non-developing pregnancy. Methodology. The study included all women admitted to the Department of obstetrics and gynecology of the Peter Great clinic with a verified diagnosis of non-developing pregnancy. In parallel, the control group included women with normal pregnancy, comparable in age and the presence or absence of comorbidities. After obtaining consent from women, peripheral venous blood was collected for genetic research. Gen CYP3A5*3/*1 (6986A>G) polymorphism was determined by real-time polymerase chain reaction method using the developed sets («Synthol», Russia). Comparison of the detected allele frequencies was performed using Chi-square method. The Graphpad Prism 5.0 statistical program was used. Results. 53 persons were included in the study group of women with non-developing pregnancy. The control group, comparable in age and comorbidity, included 92 people. The frequency of minor allele Т in the study group was 8 %, in the control group - 4 % (p = 0.1; 2). The distribution of genotypes in the main group was as follows: ТТ - 0.02; TC - 0.13; CC - 0.85. The distribution of genotypes in the control group: ТТ - 0.01; TC - 0.05; CC to 0.94. The distributions corresponded to the Hardy-Weinberg equation. Conclusion. The results of the study indicate a tendency to a higher frequency of minor allele Т in the group of women with non-developing pregnancy compared to women whose pregnancy was normal at the time of the study. The obtained data may indicate a possible role of CYP3A5 activity in the pathogenesis of non-developing pregnancy, or in the effectiveness of drug therapy, since this enzyme performs the function of endogenous metabolism of a number of sex hormones (progesterone, testosterone).