Abstract

Rhizoctonia spp. 에 의한 Rhizoctonia 마름병은 잔디에서 중요한 병이며, 골프장에서 잔디의 좋은 품질을 유지하기 위하여 살균제를 사용하고 있다. 본시험은 포장에서 분리한 R. solani AG-1 IB, R. cerealis 및 R. solani AG2-2에 대한 침투성 살균제인 flutolanil, pyraclostrobine, and hexaconazole에 대하여 실내에서 상대적인 균사생장을 효과적으로 50%억제하는 <TEX>$EC_{50}$</TEX>농도를 결정하는 시험을 수행하였다. 각각 살균제에 대한 감수성은 3종의 Rhizoctonia균주에 대하여 5 수준의 농도를 사용하였다. 3종류의 살균제에 대한 <TEX>$EC_{50}$</TEX> 값은 R. solani AG-1 IB에서 가장 낮았다. 하지만, R. solani AG2-2에 대한 살균제 감수성은 pyraclostrobine 과 flutolanil에서 평균 <TEX>$EC_{50}$</TEX> 값이 0.026 <TEX>${\mu}g\;a.i.\;ml^{-1}$</TEX> 와 0.044 <TEX>${\mu}g\;a.i.\;ml^{-1}$</TEX> 로 가장 높았다. R. cerealis은 hexaconazole에서 <TEX>$EC_{50}$</TEX> 값이 0.022 <TEX>${\mu}g\;a.i.\;ml^{-1}$</TEX>로 가장 감수성이 낮았다. 각각의 살균제의 <TEX>$EC_{50}$</TEX>값을 사용하여 두 농약을 혼용한 3종의 조합에서 3종의 병원균에 대한 균사 생장억제율은 R. solani AG2-2에서 가장 높았다. 본 연구결과는 포장에서 Rhizoctonia 마름병을 방제하기 위하여 실내에서 침투성 살균제에 대한 반응을 확인할 수 있었다. The Rhizoctonia blight causing by Rhizoctonia spp. is an important disease of turfgrass, requiring fungicide application to maintain acceptable conditions for turfgrass good qualities in the golf course. The experiment was conducted to determine the mean 50% effective concentration inhibiting mycelial growth (<TEX>$EC_{50}$</TEX>) value of to flutolanil, pyraclostrobine and hexaconazole to Rhizoctonia solani AG-1 IB, Rhizoctonia cerealis and Rhizoctonia solani AG2-2IV isolated from Gyeongbuk province of Korea in vitro. Five discriminatory concentrations of each fungicide were used to detect in vitro sensitivity. The mean of <TEX>$EC_{50}$</TEX> values to three systemic fungicides was the lowest isolate of R. solani AG-1 IB. However, the sensitivity of fungicides to Rhizoctonia solani AG2-2IV were higher mean <TEX>$EC_{50}$</TEX> value of 0.026 <TEX>${\mu}g\;a.i.\;ml^{-1}$</TEX> of pyraclostrobine and 0.044 <TEX>${\mu}g\;a.i.\;ml^{-1}$</TEX> of flutolanil. R. cerealis was the lowest sensitivity to hexaconazole which was an average <TEX>$EC_{50}$</TEX> value of 0.022 <TEX>${\mu}g\;a.i.\;ml^{-1}$</TEX>. Inhibition of mycelial growth rate (%) by three combine fungicides using the <TEX>$EC_{50}$</TEX> value of each fungicide was the highest R. solani AG2-2IV. Results of this study were may confirmed in vitro response fungicide of three Rhizoctonia species for control of Rhizoctonia blight in the field.

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