Abstract
The purpose of this study was to investigate the anti-allergic effects of a powder mixture containing ark shells (MP) on rat basophilic leukemia (RBL)-2H3 mast cells. When RBL-2H3 cells were sensitized with anti-dinitrophenyl (anti-DNP) IgE and subsequently stimulated with DNP-human serum albumin (HSA), β-hexosaminidase was released from the cells following degranulation of the activated mast cells. However, pretreatment with MP extract (MPCAS) before stimulation with DNP-HSA attenuated the release of β-hexosaminidase in IgE–antigen complex-stimulated RBL-2H3 cells. The levels of interleukin-4 (IL-4), IL-13, and TNF-α in the IgE-antigen complex-stimulated RBL-2H3 cells were measured using ELISA. Activated RBL-2H3 cells induced the expression of the abovementioned inflammatory cytokines that are critical for the pathogenesis of allergic diseases. MPCAS inhibited IL-4, IL-13, and TNF-α secretion from activated RBL-2H3 cells. In addition, the present study investigated the effects of MPCAS as an antigen on IgE-mediated activation of RBL-2H3 cells. When RBL-2H3 cells were sensitized with IgE and subsequently stimulated with MPCAS, β-hexosaminidase, IL-4, IL-13, and TNF-α levels did not change compared with those in the control. Thus, MPCAS does not act as an allergen in IgE-stimulated RBL-2H3 cells. These results indicate that MPCAS can be developed as an allergy-reducing food material by suppressing degranulation and inflammatory cytokine expression.
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