교맥의 RBL-2H3 비만세포 탈과립과 cytokine 생산 억제 효과

Abstract

Objectives : Fagopyrum esculentum(FE) has been used for removal of inflammation of internal organs and treatment of sore and ulcer by heat toxin in Korean herbal medicines. In this study, To investigated the protective effect of FE on allergic response, we determined whether FE inhibits allergic response. Methods : The effect of FE was analyzed by ELISA, RT-PCR and Western blot in RBL-2H3 cells. We investigated cell viability, <TEX>${\beta}$</TEX>-hexosaminidase, as a marker of degranulation, cytokne, and intracellular ROS and MAPK and NF-<TEX>${\kappa}B$</TEX> signaling. Results : We found that FE suppressed <TEX>${\beta}$</TEX>-hexosaminidase release, the production of IL-4 and TNF-<TEX>${\alpha}$</TEX> and intracellular ROS level in RBL-2H3 by the anti-DNP IgE plus DNP-HSA stimulation. FE also significantly inhibited cytokine mRNA expressions, such as IL-<TEX>$1{\beta}$</TEX>, IL-2, IL-3, IL-4, IL-5, IL-6, IL-13, TNF-<TEX>${\alpha}$</TEX> and GM-CSF in RBL-2H3. In addition, PF suppressed the phospholyation of ERK1/2, JNK1/2, p38 and <TEX>$I{\kappa}B{\alpha}$</TEX> and NF-<TEX>${\kappa}B$</TEX> signal transduction pathway. Conclusions : Our results indicate that FE protects against allergic response and exerts an anti-inflammatory effect through the inhibition of degranulation and production of cytokines and ROS via the suppression MAPK and NF-<TEX>${\kappa}B$</TEX> of signal transduction. Abbrevations : FE, Fagopyrum esculentum; RBL-2H3, rat basophilic leukemia cell line; ROS, reactive oxygen species; MAPK, Mitogen-activated protein kinase; <TEX>$NF{\kappa}B$</TEX>, nuclear factor <TEX>${\kappa}B$</TEX>; <TEX>$TNF{\alpha}$</TEX>, Tumor necrosis factor alpha; GM-CSF, Granulocyte macrophage colony-stimulating factor; ERK, extracellular-signal-regulated kinase; JNK, c-Jun NH2-terminal kinase; p38, p38 MAP kinase; <TEX>$I{\kappa}B{\alpha}$</TEX>, inhibitory-kappa B alpha.