봉약침액(蜂藥鍼液)이 RAW 264.7 세포의 iNOS, TNF-α 및 NF-kB에 미치는 영향(影響 )

Abstract

Objective : The purpose of this study was to investigate the effects of Bee Venom on the lipopolysaccharide(LPS), sodium nitroprusside(SNP), hydrogen peroxide-induced expression inducilble nitric oxide synthetase(iNOS), tumor necrosis factor-(TNF-) and nuclear factor kappa B(NF-kB) in RAW 264.7 cells, a murine macrophage cell line. Method : The expressions of expression iNOS and TNF- were determined by western blotting with corresponding antibodies. The expressions of expression NF-kB was assayed by EMSA method. Results : 1. The 0.5, 1 and of bee venom on LPS-induced expression of iNOS, the of bee venom on SNP-induced expression of iNOS and the of bee venom on -induced expression of iNOS compared with control were inhibited significantly. 2. The 0.5, 1 and of bee venom inhibited significantly LPS and -induced expression of TNF- compared with control, respectively. The of bee venom increased significantly SNP-induced expression of TNF- compared with control. 3. The of bee venom on LPS-induced expression of NF-kB, the of bee venom on SNP-induced expression of NF-kB and the 0.5, of bee venom on -induced expression of NF-kB were inhibited significantly compared with control, respectively.