Abstract
Bacterial blight is one of most harmful diseases of legumes, reducing the profitability of soybean production in Russian Federation. Among a number of Pseudomonas isolates obtained from diseased seeds and plants of soybean, 4 strains were selected and confirmed as Pseudomonas savastanoipv. glycinea (Psg). Properties of the isolated bacteria were similar to type strain of Psg CFBP 2214 in plant virulence, LOPAT tests, and PCR analysis for coronafacate ligase gene, and partly – in the phage reaction profile. Four isolates of bacteriophages specific to Psg were obtained from soil samples taken from fields with soybean crops. Virulence testing for the bacteriophages showed that bacteriophage ϕG17 infected 4 of 5 tested Psg strains, and it was chosen for further experiments with bacterial blight control. The bacteriophague effect control conducted on soybent plants inoculated by Psg experiments confirmed that 2 treatments of plants by the bacteriophage significantly reduced the disease development. Biological effect of the bacteriophage application was 74.75%, which is very close to the pesticide Strekar in a concentration of 0.5%.
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