Abstract
The effects of inorganic salts, inoculum concentration, aeration rate and shaking speed on insoluble phosphate solubilization by Pseudomonas fluorescens RAF15 were investigated. Soluble phosphate production was dependent on the presence of <TEX>$MgCl_2{\cdot}6H_2O$</TEX> and <TEX>$MgSO_4{\cdot}7H_2O$</TEX> in the medium. Supplementation of medium with 0.01% <TEX>$CaCl_2{\cdot}2H_2O$</TEX> and 0.01% NaCl slightly increased soluble phosphate production. The optimal medium compositions for the solubilization of insoluble phosphate by P. fluorescens RAF15 were 1.5% glucose, 0.005% urea, 0.3% <TEX>$MgCl_2{\cdot}6H_2O$</TEX>, 0.01% <TEX>$MgSO_4{\cdot}7H_2O$</TEX>, 0.01% <TEX>$CaCl_2{\cdot}2H_2O$</TEX> and 0.01% NaCl, respectively. Optimal inoculum concentration was 2.0%(v/v). Maximum soluble phosphate production was obtained with 20-50 ml/250-ml flask and 200 rpm of shaking speed, respectively. The addition of EDTA decreased cell growth and soluble phosphate production.
Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
